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KMID : 1141020120120020021
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Journal of Applied Oriental Medicine
2012 Volume.12 No. 2 p.21 ~ p.26
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Antioxidant Activities in Shoots and Roots of Hydroponic cultured Ginseng
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Park Jae-Ho
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Abstract
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Objective: Research of the antioxidant activities of Hydropoic cultured Ginseng shoots and roots extracts
Methods：Antioxidant activities of Hydropoic cultured Ginseng shoots and roots extracts were investigated using extracted various concentrations of ethylacetate (EtOAC). The DPPH, ABTS and hydroxyl radical scavenging assay and Fe2+ chelating assay were performed to characterize antioxidative effects. Also, we determinate the total phenolic content of the shoot and the root.
Results: When the different concentrations of ethyl acetate (0.32, 1.6, 8, 40, 200㎍/㎖) were tested with the shoot of Hydropoic cultured Ginseng, the extracts at 200 ug/ml resulted in the highest DPPH, ABTS, hydroxyl radical scavenging, and Fe2+ chelating activities, and the activities were 81, 86, 41, and 52%, respectively. The shoot extracts showed the higher antioxidative activities than the root extracts at same concentration. The total phenolic contents of shoot and root were 31.3 mg/g, 25.1 mg/g, respectively. We consider that the phenolic compound is associated with antioxidant activities.
Conclusion: This study indicates that the shoot extracts showed higher antioxidant activities and total phenolic content than the root extracts.
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KEYWORD
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Hydropoic cultured Ginseng, antioxidant activity, DPPH, ABTS, Hydroxyl radical Fe2+ chelating
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